anti‐Sarcoptes antibodies in serum. Positive results indicate possible Sarcoptes infection, or false positive due to house dust mite hypersensitivity (these antigens cross react). Dogs may remain positive for up to 6 months after successful treatment. Negative results indicate lack of exposure, early infection, or immunosuppressive medication interactions. Sensitivity is 84.2%, and specificity is 89.5%
http://www.idexx.com
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Tick PCR Panels
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Antech: Tick Borne PCR Panel
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Whole blood (EDTA)
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Indicates the presence of DNA from various tick‐borne organisms
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www.antechdiagnostics.com
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Cornell University College of Veterinary Medicine, Animal Health Diagnostic Center PCR
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Indicates the presence of DNA from Ehrlichia canis, Neorickkettsia risticii, Anaplasma phagocytophilum, Bartonella spp., hemotropic Mycoplasma spp. Many additional individual PCR tests available on website
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http://www.vet.cornell.edu/animal‐health‐diagnostic‐center/testing/protocols/blood‐parasite
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IDEXX: Tick/Vector Comprehensive RealPCR Panel
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Indicates the presence of DNA from Anaplasma spp., Babesia spp., Bartonella spp., canine hemotropic Mycoplasma, Ehrlichia spp., Hepatozoon spp., Leishmania spp., Neorickettsia risticii, and Rickettsia rickettsii. Quantifies DNA of Leishmania spp.
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www.idexx.com
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North Carolina State University Vector Borne Disease Diagnostic Laboratory
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Indicates the presence of DNA from Bartonella spp., Anaplasma spp., Ehrlichia spp., Babesia spp., Cytauxzoon felis, Mycoplasma spp., Leishmania spp., Rickettsia spp.
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http://cvm.ncsu.edu/research/labs/clinical‐sciences/vector‐borne‐disease
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Tick Serology Panels
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Antech: AccuPlex™ 4 Fastpanel PCR Canine Tick Borne Profile w/Lyme
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Serum Serum Whole blood (EDTA)
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Measures antibodies to Borrelia burgdorferi, Anaplasma sp., and Ehrilichia sp., and measures Dirofilaria immitis antigen Measures presence of DNA from several tick‐borne organisms, and measures Borrelia burgdorferi antibodies. Also offers B. burgdorferi PCR and Western Blot
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www.antechdiagnostics.com
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Cornell University College of Veterinary Medicine, Animal Health Diagnostic Center
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Serum
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Measures antibodies to Rickettsia rickettsii, Neorickettsia risticii, Ehrlichia canis, Brucella canis, Bartonella spp. by IFA; measures antibodies to Borrelia burgdorferi, Ehrlichia canis, and Anaplasma spp. by ELISA; measures Leishmania infantum by indirect ELISA
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http://www.vet.cornell.edu/animal‐health‐diagnostic‐center/testing/protocols/blood‐parasite
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IDEXX: Tick Panel – immunofluorescence antibody (IFA) test Lab 4Dx® Plus Test (ELISA) Lyme Quant C6®
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Serum Serum Serum Urine
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Quantifies Ehrlichia canis, Rickettsia rickettsii, and Borrelia burgdorferi antibody Measures antibodies to Anaplasma phagocytophilum, Anaplasma platys, Ehrlichia canis, Ehrlichia ewingii, and Borrelia burgdorferi, and presence of Dirofilaria immitis antigen Measures Borrelia burgdorferi antibody, limited chemistry profile, limited complete blood count, SDMA, urinalysis, and urine protein:creatinine ratio. Lyme Quant C6 testing is available with the RealPCR panel.
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www.idexx.com
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North Carolina State University Vector Borne Disease Diagnostic Laboratory
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Serum
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Measures antibodies to Bartonella hensalae, Bartonella vinsonii, Bartonella koehlerae, Babesia canis, Babesia gibsonii, Ehrlichia canis, Rickettsia rickettsii, Leishmania infantum, Anaplasma sp., Borrelia burgdorferi
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http://cvm.ncsu.edu/research/labs/clinical‐sciences/vector‐borne‐disease
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Bacterial PCR
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Provided by many research institutions and universities. Polymerase chain reaction (PCR) tests are available for common dermatologically relevant bacteria, including Staphylococcus spp., Pseudomonas sp., Streptococcus sp., E. coli, Bordetella sp., Mycobacteria spp., Bartonella sp., etc. Refer to diagnostic laboratories at large research institutions nearest you for more information, especially in regard to each test's sensitivity and specificity. While most PCRs are highly sensitive and specific, remember that the presence of DNA can represent contamination of the sample or the presence of nonviable bacteria. Results should be correlated with clinical findings, cytology, histopathology, and even culture when possible
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Fungal serology
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IDEXX
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Serum
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Measures antibodies to Coccidioides sp., Blastomyces sp., Histoplasma sp., Aspergillus sp.; measures Cryptococcus sp. antigen (latex agglutination)
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www.idexx.com
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Antech
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Serum Urine
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Measures antibodies to various fungi, including Coccidioides sp., Histoplasma sp., and others. Measures presence of Cryptococcus sp. antigen (latex agglutination) and Blastomyces sp. antigen (urine)
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www.antechdiagnostics.com
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Provided by many research institutions and universities. PCRs are available for common dermatologically relevant fungi. Refer to diagnostic laboratories at large research institutions nearest you for more information, especially in regard to each test's sensitivity and specificity
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Fungal PCR
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IDEXX: Blastomyces dermatitidis RealPCR Test Systemic Mycoses RealPCR Test
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Whole blood (EDTA) or deep pharyngeal swab Aspirated material or fresh tissue
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Positive indicates the presence of Blastomyces dermatitidis DNA in sample and must be considered with presence of clinical signs consistent with infection. False negative is possible if sample is inadequate or due to antifungal therapy administration Positive indicates presence of DNA from Blastomyces dermatitidis, Coccidioides spp.,
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