Chapter 12
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Gel and capillary electrophoresis
|
Chapter 10
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|
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Next generation sequencing
|
Chapter 14
|
|
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Microsatellite analysis
|
Chapter 11
|
|
|
SNP analysis
|
Chapters 14 and 21
|
|
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FISH
|
Chapter 11
|
|
|
In situ hybridization
|
Chapter 11
|
|
RNA (transcriptomics)
|
RNA‐seq (NGS)
|
Chapters 14 and 21
|
|
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DNA microarrays
|
Chapter 11
|
|
|
In situ hybridization
|
Chapter 11
|
|
Cell and tissue culture
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Cells with reporter genes
|
|
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Cell sorting
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|
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Organoid cultures
|
|
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Stem cells
|
|
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Cancer cells
|
|
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Hybridoma cells for production of monoclonal antibodies
|
|
|
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Cell cycle analysis
|
Chapter 18
|
|
|
Patch clamp recording
|
Chapter 17
|
|
Microscopy
|
Light microscope (bright field, dark field, phase contrast, differential interference contrast)
|
Chapter 19
|
|
|
Fluorescence microscope (confocal)
|
Chapters 19 and 20
|
|
|
Immunofluorescence and GFP fusion proteins
|
Chapter 19
|
|
|
Super‐resolution microscopy (STED, SIM, PALM, STORM)
|
Chapter 19
|
|
|
Atomic force microscopy
|
Chapter 19
|
|
|
Electron microscope
|
Chapter 19
|
|
|
Scanning electron microscope (SEM)
|
|
|
|
Cryoelectron microscopy
|
Chapter 19
|
|
|
Image processing
|
|
|
Cloning and expression
|
Plasmid and viral vectors
|
Chapter 15
|
|
|
Expression vectors
|
Chapters 15 and 16
|
|
|
Fermenters
|
|
|
|
Genomic and cDNA libraries
|
Chapter 21
|
|
|
Reverse genetics
|
|
|
Genetic engineering
|
Transformation
|
Chapter 15
|
|
|
Transfection
|
Chapter 15
|
|
|
RNAi
|
|
|
|
CRISPR–Cas gene editing
|
|
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Transgenic organism
|
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