extract of A. marmelos (Mohammed et al. 2016).
2.5.2 Culture Conditions
The cell culture studies of A. marmelos were established on MS (Murashige and Skoog 1962) culture medium with supplementation of BAP + 2,4-D (2,4-Dichlorophenoxyacetic acid) and IAA, NAA, etc., for estimation of flavonoids. The maximum accumulation of flavonoids was reported in the callus fed with IAA, NAA, and NaCl and KCl (Islam et al. 1995; Soni and Goswami 2013). The organ differentiation with BAP in MS medium, the changes in accumulation of fatty acid, phospholipids, and glycolipid were reported. The maximum number shoots and weight were obtained after eight weeks of culture on MS medium supplemented with BAP (Hazarika et al. 1996). Similarly, the genetic transformation is a useful strategy in enhancing the quantity of marmelosin like bioactive compounds in A. marmelos (Pati and Muthukumar 2013).
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