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Dentin is deposited by odontoblasts that develop ectomesenchymal cells of the dental papilla on contact with the basal lamina formed by the inner enamel epithelium.
Differentiation of Odontoblasts
Odontoblast precursors migrate into the developing jaw from the neural crest as part of a large population of ectomesenchymal cells that participate in the formation of many parts of the face and oral cavity. During the cap stage of tooth formation, the preodontoblasts concentrate adjacent to the inner enamel epithelium (IEE) of the enamel organ. Preodontoblasts exit the cell cycle and differentiate before the preameloblasts of the IEE have stopped dividing.1,2
Contact with the IEE basement membrane and/or with other associated extracellular material of epithelial origin has long been held to be a requirement for initial odontoblastic differentiation.3 Recent experiments suggest that a fibronectin-rich substratum is a key requirement.4 Early studies implicating the importance of the basement membrane in odontoblast differentiation were reviewed by Ruch1,2 and Ruch et al.5
Aperiodic fibrils are key structures regulating the differentiation of odontoblasts. They are deposited first at the tip of the future