Gregory T. Benz

Agitator Design for Gas-Liquid Fermenters and Bioreactors


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      Flooding is a condition in which there is too much gas flowing into an impeller for it to effectively disperse it. If the main purpose of the agitator is to disperse the gas, this is obviously not a desirable condition. But how do we define it quantitatively and how do we measure it? There are actually several ways. We will describe three of them here.

      By kla, we mean the overall mass transfer coefficient, as defined by:

      (4.1)equation

Graph depicts the plot of flooding determination by using kla.

      One could also use mass transfer rate as the y‐axis if the driving force remained constant.

      While this definition of flooding has the advantage of clearly defining the point at which the agitator actually begins to influence mass transfer and thus relates to the main purpose for the use of the agitator, it is experimentally difficult to carry out. Most published definitions and correlations of flooding do not use this method, as we will see. This method does, however, have the advantage of being possible to carry out in an opaque vessel.

Graph depicts impeller flooding by power draw.

      Condition (c) in Figure 4.3 is called complete dispersion. It occurs when gas is driven to the bottom of the tank and some of it is recirculated into the impeller. It is not a design condition per se, but usually occurs when agitation is sufficient to support medium and high mass transfer rates (for example, above 80 mmol/l‐h for an air‐based fermentation at a back pressure of 1 bar‐g or less.)

      The transitions from condition (a) through condition (c) can be thought of as either increasing shaft speed at constant airflow or decreasing airflow at constant shaft speed.

Schematic illustration of visual flooding.

      As mentioned in the first section on flooding, when gas is introduced to a liquid, it affects power draw. At very low gas rates, some impellers actually have an increased power draw. Likely, this is due to increasing the discharge head imposed on the impeller. But at most gas flowrates used in fermenters and bioreactors, gas causes the power to drop.

      When gas is dispersed into liquids, it does not rise to the surface instantly. Smaller bubbles take longer to discharge at the surface than larger ones. High power/volume produces smaller bubbles, on average. And the more gas is introduced, the more that will be present in the broth at any given moment. Moreover, as the agitation gets stronger, there is more recirculation of bubbles. All of these things contribute to gas holdup.

      From a fluid property point of view, surface tension, ionic strength,