Martinez J. Hewlett

Basic Virology


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Cells lose their requirement for anchorage to a surface during growth. Also lost is contact inhibition. As a result, cells grow over each other and also grow as a suspension culture. Oncornaviruses Vacuolization Proliferation of cytoplasmic vacuoles late during the infectious cycle Flaviviruses Cell fusion Cell–cell fusion leading to the formation of syncytium and polykaryons Paramyxoviruses Inclusion bodies Densely staining structures within the cytoplasm or nucleus of the cell; often indicates location of viral assembly Various virus families Apoptosis Programmed cell death, characterized by nuclear events leading to chromosomal disaggregation Lentiviruses

      Virus exit is essentially the converse of virus entry at the start of the infection. Now, however, the infected individual is a reservoir of the continuing infection, and symptoms of the disease may have a role in its spread. Some examples should illustrate this simple concept. Infection with a mosquito‐borne encephalitis virus results in high titers of virus in the victim's blood. At the same time, the infected individual's malaise and torpor make him or her an easy mark for a feeding mosquito. In chickenpox (caused by herpes zoster virus, also called varicella zoster virus[VZV]), rupture of virus‐filled vesicles at the surface of the skin can lead to generation of viral aerosols that transmit the infection to others. Similarly, a respiratory disease–causing virus in the respiratory tract along with congestion can lead to sneezing, an effective way to spread an aerosol. A virus such as HIV in body fluids can be transmitted to others via contaminated needles or through unprotected sexual intercourse, especially anal intercourse. Herpesvirus in saliva can enter a new host through a small crack at the junction between the lip and the epidermis.

      Following a viral or any infectious disease, the host recovers or dies. While many acute infections result in clearance of virus, this does not invariably happen. While infections with influenza virus, cold viruses, polioviruses, and poxviruses resolve with virus clearance, herpesvirus infections result in a lifelong latent infection. During the latent period, no infectious virus is present, but viral genomes are maintained in certain protected cells. Periodically, a (usually) milder recurrence of the disease (reactivation or recrudescence) takes place upon suitable stimulation.

      In distinct contrast, measles infection resolves with loss of infectious virus, but a portion of the viral genome can be maintained in neural tissue. This is not a latent infection because the harboring cells can express viral antigens, which lead to lifelong immunity, but infectious virus can never be recovered.

      Other lasting types of virus‐induced damage can be much more difficult to establish without extensive epidemiological records. Chronic liver damage due to hepatitis B virus infection is a major factor in hepatic carcinoma. Persistent virus infections can lead to immune dysfunction. Virus infections may also result in the appearance of a disease or syndrome (a set of diagnostic signs and symptoms displayed by an affected individual) years later that has no obvious relation to the initial infection. It has been suggested that diseases such as diabetes mellitus, multiple sclerosis, and rheumatoid arthritis have viral etiologies (ultimate causative factors). Virus factors have also been implicated in instances of other diseases such as cancer and schizophrenia.

      1 A good general rule concerning the replication of RNA viruses is that they require what kind of molecular process?

      2 What is the role of a vector in the transmission of a viral infection?

      3 It is said that viruses appear to “violate the cell theory” (“cells only arise from preexisting cells”). To which phase of a virus life cycle (growth curve) does this refer? What is the explanation for this phase of the growth curve?

      4 Viruses are called “obligate intracellular parasites.” For which step of gene expression do all viruses completely depend on their host cell?

      5 Viruses are said to “violate the cell theory,” indicating that there are differences between viruses and cells. The following table lists several features of either viruses or cells or both. Indicate which of these features is true for viruses and which for cells. In each case, write a “Yes” if the feature is true or a “No” if the feature is not true.FeatureCellsVirusesThe genetic information may be RNA rather than DNA.New individuals arise by binary fission of the parent.Proteins are translated from messenger RNAs.New individuals assemble by spontaneous association of subunit structures.

      6 At this writing, the avian influenza H5N1 is transmitted from bird to bird, although it may, at some point, mutate to allow transmission from human to human. What feature of virus–host interaction does this characterize?

        THE NATURE OF VIRUS RESERVOIRS

        Some viruses with human reservoirs

        Some viruses with vertebrate reservoirs

        VIRUSES IN POPULATIONS

        Viral epidemiology in small and large populations

        Factors affecting the control of viral disease in populations

        ANIMAL MODELS TO STUDY VIRAL PATHOGENESIS

        A mouse model for studying poxvirus infection and spread

        Rabies: Where is the virus during its long incubation period?

        Herpes simplex virus latency Murine models Rabbit models Guinea pig models

        QUESTIONS FOR CHAPTER 3