3 Marine Polysaccharides: Properties and Applications
Tonmoy Ghosh, Rabinder Singh, Asha Arumugam Nesamma and Pannaga Pavan Jutur*
Omics of Algae Group and DBT-ICGEB Centre for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India
Abstract
Polysaccharides arguably are the most diverse group of biopolymers on earth with critical roles in energy storage, cellular structure components and safekeeping of hereditary information. With the wide variety of structures, source organisms and years of evolutionary history in different habitats, these biomolecules encompass an array of derivatizations and conjugation with other biomolecules. This chapter would mainly focus on the major polysaccharides obtained from the marine environment, i.e. crustaceans, seaweeds, and microalgae. The polysaccharides will be discussed in context of their various properties and the applications for which they are or can be exploited in industries such as biomedical, pharmaceutical, agriculture, and food. This chapter summarizes the diversity of polysaccharides with the underlying basic principles and their versatile applications. Keywords: Alginate, cellulose, chitosan, crustaceans, marine polysaccharides, seaweed 3.1 Introduction Marine world is an ideal model for studying species diversity. The main reasons for this is the abundant availability of water, presences of a large area favorable for photosynthesis, and lesser temperature variations compared to land climatic conditions [1, 2]. The wide species diversity can be broadly attributed to variable primary productivity in different ocean zones according to light availability [3]. Members of different species therefore evolved differently based on the biological functions that are suitable for its own habitat; this led to a rise in different biomolecules sharing a common structure existing across species [4] but differing in the basic properties according to the requirements. Although this holds true for many biomolecules present today, nothing displays the extent of variation occurring in polysaccharides. Their essential nature coupled with more than 2.5 billion years of evolutionary history simultaneously resulted in organisms employing this molecule for many key functions. Although not different from other polymers (proteins, lipids), polysaccharides are the most studied, utilized and information dense on earth, with a common basic structure but with a multitude of derivatizations [5, 6]. Polysaccharides are high-molecular polymers, also called glycans, made up of repeated units of monosaccharides and bound to unique glycoside bonds. They are omnipresent in the biological world, with roles as diverse as energy storage, building the cellular framework, to encoding genetic information through generations [6]. They can be water soluble or insoluble, and their structural diversity stems from different derivatization reactions taking place. On the basis of monomeric units, they can be divided in to 2 categories: homopolysaccharide (composed of same monomers), and heteropolysaccharide (composed of 2 or more different monomers). However, due to the diversity of the polysaccharide family, a more general classification is based on the intended functions: Storage polysaccharides (glycogen, starch) Structural polysaccharides (chitin, cellulose) Hereditary (DNA, RNA); conjugated with nitrogenous bases. Structurally, polysaccharides can be linear (cellulose, agar) or branched (amylopectins, arabinans) [7]. Marine polysaccharides are essentially derived from marine organisms, like algae, seaweeds, bacteria, corals, sponges, fungi, and crustaceans (organisms like shrimps, crabs, prawns, and lobsters). This chapter addresses the properties and major applications of polysaccharides from marine sources in biomedical, food, pharmaceutical and agrarian industries. 3.2 Polysaccharide Origins Polysaccharides, being essential to all life forms, are found in a variety of organisms in different forms. Marine polysaccharides are mainly derived from crustaceans (shrimps, crabs), seaweeds, bacteria, and fungi. Table 3.1 lists the major polysaccharides along with the sources, monomers and type of linkages. In addition, the following sections will address these molecules in more detail. 3.3 Properties Polysaccharides are found in almost all living organisms, residing typically in a wide variety of habitats. The heterogeneity of polysaccharides and alternative methods of derivatization across time has given rise to several candidates with a broad spectrum of physicochemical properties. In this chapter of marine polysaccharides, we will concentrate on a few of candidate biopolymers. 3.3.1 Cellulose Cellulose is a very significant member of the polysaccharide family because of its presence in plant and algal cell walls. Some species of the bacteria also produce cellulose, such as Acetobacter sp., Komagataeibacter sp., and Agrobacterium sp. The main difference between celluloses from plant and bacterial origins are the absence of hemicellulose and lignin in the bacterial type, thus avoiding the need for harsh pre-treatment procedures commonly used for cellulose purification among the plant systems [8, 9]. Table 3.1 Types, sources, monomers, and linkages found in common polysaccharides.
Type
Sources
Monomers
Linkage
References
Cellulose
Seaweed, bacteria
Glucose
β(1→4)
[8, 10]
Chitosan
Shrimps, crabs, fungi
N-acetyl glucosamine, glucosamine
β(1→4)
[13]
Alginate
Seaweed
β-D-mannuronate, α-L-guluronate
β(1→4)
[208]
Carrageenan
Seaweed
Galactose, 3,6-anhydrogalactose, ester bound sulfate
–
[208]
Agar
Seaweed
3,6-anhydro-α-lgalactopyranose, β-dgalactopyranose
–
[208]
Porphyran
Seaweed
β-D-galactose, α-Lgalactose, 3,6-anhydro- α-L-galactose
[40]
Fucoidan
Seaweed
