Richard J. Miron

Understanding Platelet-Rich Fibrin


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the highest concentration of liquid-PRF, as discussed further in chapter 5. Nevertheless, based on the C-PRF protocols established in chapter 2, it remained obvious that better concentrations could be achieved by further modifying centrifugation parameters.

      Establishing C-PRF on a Horizontal Centrifuge

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      Optimization of C-PRF protocols

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      Tips

       In clinical practice, it is best to harvest C-PRF by first removing the upper 3 to 4 mL of platelet-poor plasma (discard it). The remaining C-PRF layer can then be taken much more easily. It is much more difficult to attempt to retrieve this buffy coat zone with 5 mL over top of it; it is harder to concentrate it, and too much volume is often collected.

       Many centrifuges may not reach the 3000g speed. A 2000g protocol for 8 minutes will also achieve a C-PRF layer.

      Comparative GF release between i-PRF and C-PRF

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      Biocompatibility and cellular activity of i-PRF and C-PRF

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