Understanding Platelet-Rich Fibrin. Richard J. Miron

mean ± SE; an asterisk (*) indicates a value significantly higher than the control group (P < .05); a double asterisk (**) indicates a value significantly higher than all other groups (P < .05); a number sign (#) indicates a value significantly lower than all groups (P < .05). (Reprinted with permission from Fujioka-Kobayashi et al.¹¹)

      Conclusion

      In summary, the results from the 24-protocol investigation demonstrated clearly that certain protocols were better at increasing the amount of platelet/leukocyte yield (400g to 700g for 8 minutes), whereas others were more effective at concentrating PRF (200g to 300g for 5 minutes). Therefore, new protocols were designed accordingly for solid-PRF and liquid-PRF. While histologically it was observed that horizontal centrifugation led to better cell layer separation of blood cells as well as more even distribution of cells throughout the PRF clot, it was also histologically observed that the majority of cells accumulated on the back distal surfaces of PRF tubes when fixed-angle centrifugation was carried out. Lastly, a new C-PRF formulation was evaluated demonstrating up to a threefold increase in GF release during a 10-day period and further elicited fourfold increases in gingival fibroblast migration, PDGF gene expression, and collagen1 synthesis when compared to standard i-PRF protocols.

      References

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