Lynne Shore Garcia

Practical Guide to Diagnostic Parasitology


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href="#ub66d1a7d-3ea6-47a7-a11f-d12dc5ed0c1c">Overview of Preservatives

       Formalin

       Sodium Acetate-Acetic Acid-Formalin (SAF)

       Schaudinn’s Fluid

       Polyvinyl Alcohol (PVA)

       Modified PVA (Mercury Substitutes)

       Single-Vial Collection Systems (Other Than SAF)

       Quality Control for Preservatives

       Procedure Notes for Use of Preservatives (Stool Fixative Collection Vials)

       Procedure Limitations for Use of Preservatives (Stool Fixative Collection Vials)

       Collection of Blood

       Collection and Processing

       STAT Test Requests and Risk Management Issues

       Collection of Specimens from Other Body Sites

       Table 3.1 Fecal Specimens for Parasites: Options for Collection and Processing

       Table 3.2 Approaches to Stool Parasitology: Test Ordering

       Table 3.3 Preservatives and Procedures Commonly Used in Diagnostic Parasitology (Stool Specimens)

       Table 3.4 Advantages of Thin and Thick Blood Films

       Table 3.5 Advantages and Disadvantages of Buffy Coat Films

       Table 3.6 Potential Problems of Using EDTA Anticoagulant for the Preparation of Thin and Thick Blood Films

       Table 3.7 Body Sites and Possible Parasites Recovered

       SECTION 4 Specimen Test Options: Routine Diagnostic Methods and Body Sites

       Ova and Parasite Examination of Stool Specimens

       Other Diagnostic Methods for Stool Specimens

       Culture of Larval-Stage Nematodes

       Estimation of Worm Burdens through Egg Counts

       Hatching Test for Schistosome Eggs

       Screening Stool Samples for Recovery of a Tapeworm Scolex

       Testing of Other Intestinal Tract Specimens

       Examination for Pinworm

       Sigmoidoscopy Material

       Duodenal Drainage Material

       Duodenal Capsule Technique (Entero-Test)

       Urogenital Tract Specimens

       Sputum

       Aspirates

       Biopsy Specimens

       Blood

       Thin Blood Films

       Thick Blood Films

       Blood Staining Methods

       Buffy Coat Films

       QBC Microhematocrit Centrifugation Method

       Knott Concentration

       Membrane Filtration Technique

       Culture Methods

       Animal Inoculation and Xenodiagnosis

       Antibody and Antigen Detection

       Antibody Detection

       Antigen Detection and Nucleic Acid-Based Tests

       Intradermal Tests

       Table 4.1 Body Site, Procedures and Specimens, Recommended Methods and Relevant Parasites, and Comments

       Table 4.2 Serologic, Antigen, and Probe Tests Used in the Diagnosis of Parasitic Infections