Clonorchis sinensis (Opisthorchis sinensis)
Schistosoma spp. (S. mansoni, S. haematobium, S. japonicum, S. mekongi, S. intercalatum)
Identification Keys 8.1 to 8.4
Figures 8.1 to 8.3
Preface
As we move into the 21st century, the field of diagnostic medical parasitology continues to see some dramatic changes, including newly recognized pathogens, the spread of familiar pathogens, successes and failures related to disease control, geographic and climate changes that support the spread of parasitic disease, new methodology, regulatory requirements that impact on diagnostic testing, and an overall review of the approach to and clinical relevance of this type of diagnostic testing on patient care within the managed care environment, as well as the world as a whole.
The second edition of the Practical Guide to Diagnostic Parasitology is organized to provide maximum help to the user, particularly from the bench use perspective. New aspects of the field have been addressed in these sections, and many new figures and plates have been added. Section 1 on the philosophy and approach to diagnostic parasitology has been expanded to include discussions on the possible impact of global warming, population movements, potential outbreak testing, the development of laboratory test menus, and the risk management issues related to STAT testing. The discussion of organism classification and relevant tables has been expanded to provide the user with current information related to changes in nomenclature and overall importance of the various parasite categories to human infection.
In Section 3, expanded information on stool specimen fixatives and testing options has been provided. This information is valuable for any laboratory reviewing collection and testing options related to fixative compatibility with the routine ova and parasite examination, as well as the newer fecal immunoassays. Although some fecal immunoassay reagents are now commercially available for Enterocytozoon bieneusi and Encephalitozoon intestinalis, they are not FDA approved; always check the literature from the relevant company to confirm the FDA status of any new product. The discussion on blood collection, including the pros and cons of current changes from finger stick blood to venipuncture, has been greatly enhanced, particularly related to potential problems with blood parasite morphology and lag time issues. Additional tables serve to summarize much of this new information.
Additional tables and information have been added to Sections 4 and 5, including a number of new algorithms. Section 6 is one of the most important sections in the book, with extensive revisions related to the most commonly asked questions regarding diagnostic parasitology methods. Additional techniques have been included, as well as new information related to reporting results and the importance of report comments. This section format makes it easy for the reader to use the expanded information.
Section 7 has been greatly expanded; information on each organism has been formatted on facing pages for easy access and reference. Figures have also been expanded and updated. Section 8 on identification aids has been expanded with additional tables and new plates. All of the changes for this edition are based on the need for the readers to update their information related to diagnostic medical parasitology and specifically the issues mentioned below.
With continued emphasis on regulatory requirements related to chemical disposal and the use of mercury compounds, laboratories are being required to develop skills using substitute fixatives that are prepared without the use of mercury-based compounds. Although continued work with these substitute fixatives has not produced the exact same quality organism morphology, the more relevant question is whether the intestinal parasites can be identified by using these alternative fixatives. In most cases, organism identification is comparable; an example of a rare exception is one in which the number of organisms present is quite low. This is a prime example of a change where “different” has been acceptable and relevant, not necessarily “good” or “bad.”
Many laboratories are reviewing all microbiological services, and some specific questions are being asked related to diagnostic parasitology options. Some of these questions include the following: what laboratories should be performing this type of testing, when should testing be performed, what tests should be performed, and what factors should be considered when developing test menus.
Laboratories are also reviewing specimen collection options, particularly as they relate to their geographic area and types of patients serviced. This kind of analysis is beneficial to all concerned, not only in helping laboratories to understand the specimen collection options, but how they relate to test orders, diagnostic testing, and results impacting patient care.
With changes in collection, testing, reporting, and interpretation options, it is critical to remember that this information needs to be shared with the laboratory’s client base, particularly if the test orders and results are to be used for the best-quality patient care. Although there are many ways to approach diagnostic parasitology testing, it is mandatory that the laboratory and user both understand the pros and cons of the methods selected. The use of different approaches to parasitology diagnostic testing is acceptable; however, the benefits and drawbacks must be thoroughly understood by all participants. There may be legitimate reasons why different approaches are used by different laboratories; however, cost containment must not be the sole factor in selecting methods.
Another consideration is the fact that not all clinical laboratories will continue to perform diagnostic parasitology testing. This may be due to financial considerations, lack of skilled personnel, etc. With increased emphasis on cross-trained individuals, the technical expertise required to identify these parasites by using routine microscopy may be lacking. Even with the use of molecular diagnostics, these tests are not capable of covering the entire spectrum of organisms that may be present as pathogens. However, as more of these methods become commercially available, the use of nonmicroscopic methods will increase in scope. Many laboratories now include both the ova and parasite examination and various fecal immunoassays on their routine test menus; on the basis of patient histories and symptoms, appropriate orders may focus on one or the other of these options. An important consideration in deciding whether to send out parasitology testing or maintain the testing in-house relates to STAT testing (collection, processing, testing, reporting of thick and thin blood films, and the examination of cerebrospinal fluid and other specimens for the presence of free-living amebae). These tests must be handled as STATs; the time required from collection to reporting must be considered prior to moving these procedures off-site.
Based on the many changes in