Lynne Shore Garcia

Practical Guide to Diagnostic Parasitology


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SECTION 5 Specific Test Procedures and Algorithms

       Microscopy

       CALIBRATION OF THE MICROSCOPE

       Ova and Parasite Examination

       DIRECT WET FECAL SMEAR

       SEDIMENTATION CONCENTRATION (Formalin-Ethyl Acetate)

       FLOTATION CONCENTRATION (Zinc Sulfate)

       PERMANENT STAINED SMEAR

       Stains Used in the Permanent Stained Smear

       TRICHROME STAIN (Wheatley’s Method)

       IRON HEMATOXYLIN STAIN (Spencer-Monroe Method)

       IRON HEMATOXYLIN STAIN (Tompkins-Miller Method)

       MODIFIED IRON HEMATOXYLIN STAIN (Incorporating the Carbol Fuchsin Step)

       POLYCHROME IV STAIN

       CHLORAZOL BLACK E STAIN

       Specialized Stains for Coccidia and Microsporidia

       KINYOUN’S ACID-FAST STAIN (Cold Method)

       MODIFIED ZIEHL-NEELSEN ACID-FAST STAIN (Hot Method)

       CARBOL FUCHSIN NEGATIVE STAIN FOR CRYPTOSPORIDIUM (W. L. Current)

       RAPID SAFRANIN METHOD FOR CRYPTOSPORIDIUM (D. Baxby)

       RAPID SAFRANIN METHOD FOR CYCLOSPORA, USING A MICROWAVE OVEN (Govinda Visvesvara)

       AURAMINE O STAIN FOR COCCIDIA (Thomas Hänscheid)

       MODIFIED TRICHROME STAIN FOR MICROSPORIDIA (Weber, Green Counterstain)

       MODIFIED TRICHROME STAIN FOR MICROSPORIDIA (Ryan, Blue Counterstain)

       MODIFIED TRICHROME STAIN FOR MICROSPORIDIA (Evelyn Kokoskin, Hot Method)

       Fecal Immunoassays for Intestinal Protozoa

       Entamoeba histolytica

       Cryptosporidium spp.

       Giardia lamblia

       Kits under Development

       Comments on the Performance of Fecal Immunoassays

       Larval Nematode Culture

       HARADA-MORI FILTER PAPER STRIP CULTURE

       BAERMANN CONCENTRATION

       AGAR PLATE CULTURE FOR STRONGYLOIDES STERCORALIS

       Other Methods for Gastrointestinal Tract Specimens

       EXAMINATION FOR PINWORM (Cellulose Tape Preparations)

       SIGMOIDOSCOPY SPECIMENS (Direct Wet Smear)

       SIGMOIDOSCOPY SPECIMENS (Permanent Stained Smear)

       DUODENAL ASPIRATES

       Methods for Urogenital Tract Specimens

       RECEIPT OF DRY SMEARS

       DIRECT SALINE MOUNT

       PERMANENT STAINED SMEAR

       URINE CONCENTRATION (Centrifugation)

       URINE CONCENTRATION (Nuclepore Membrane Filter)

       Preparation of Blood Films

       THIN BLOOD FILMS

       THICK BLOOD FILMS

       COMBINATION THICK-THIN BLOOD FILMS

       BUFFY COAT BLOOD FILMS

       Blood Stains

       GIEMSA STAIN